Kinorhyncha COX1 sequences from Disko Island, Greenland
Material for the present study was collected from twelve sampling stations in August 3-9, 2023, during an expedition to Disko Island (Western Greenland), organised by the Natural History Museum of Denmark (NHMD). Sediment samples were collected with a dredge and/or a Van Veen grab. Kinorhynchs were extracted from the sediment, sorted under a stereomicroscope, preserved in 99 % EtOH and kept in −20 ◦C until further analysis. Single individuals were selected for DNA extraction using Tissue DNA Purification Kit (EURx, Gdansk, Poland) with additional step of cuticle retention (centrifugation of lysis buffer at 11,000×g, followed by separation of exoskeleton from supernatant including eluted DNA). The exoskeletons were recovered, transferred to a microscopic glass slide and mounted in Fluoromount-G for light microscopy (LM). The exoskeletons were examined using a Nikon 600 and an Olympus BX51 equipped with an Olympus SC300 camera, catalogued and deposited in NHMD. DNA samples were used for fragment cox1 gene PCR amplification using LCO1490 and HCO2198, forward and reverse primer pairs with final concentration 0.2 μM, respectively (Folmer et al., 1994). Amplification reaction was carried out using onHybrid PCR Master Mix (EURx, Gdansk, Poland) with the protocol consisting of: preliminary denaturation (5 min, 95 ◦C), 35 cycles (denaturation - 30 s, 95 ◦C; annealing - 30 s, 49 ◦C; extension - 90 s, 72 ◦C) and final extension (5 min, 72 ◦C). Samples were purified with PCR/DNA Clean-Up Purification Kit (EURx, Gdansk, Poland) and sent for EZ-Seq sequencing (Macrogen Europe). Obtained sequences were assembled into contigs, trimmed and analyzed using SeaView version 5.0.5 software (Gouy et al., 2010) and deposited in NCBI GenBank database with their assigned accession numbers.
Simple
- Date (Publication)
- 2024-09-08
- Purpose
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report
- Point of contact
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Organisation name Individual name Electronic mail address Role Institute of Oceanology
Aleksandra Zalewska
Point of contact
- Maintenance and update frequency
- As needed
- Metadata author
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Organisation name Individual name Electronic mail address Role Institute of Oceanology
Publisher
- Theme
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Greenland
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- Keywords
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molecular biology parameters
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Continents, countries, sea regions of the world.
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Greenland
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- Access constraints
- License
- Use constraints
- License
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CCBY
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- Text, table
- Language
- English
- Character set
- UTF8
- Topic category
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- Environment
- Biota
- Begin date
- 2023-08-03
- End date
- 2024-10-22
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- Supplemental Information
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The data was used in published study: Zalewska, A., Herranz, M., Lubośny, M., Sørensen, M. V., & Grzelak, K. (2024). To be, or not tu-be? Population structure and connectivity in three Arctic kinorhynch species: Echinoderes aquilonius, Echinoderes eximus and Echinoderes remanei. Zoologischer Anzeiger, 313, 289-297. https://doi.org/10.1016/j.jcz.2024.10.0 13
The study was completed thanks to funding provided by the National Science Centre, Poland (grant no. 2022/45/B/NZ8/03545) to KG. The fieldwork in Greenland was supported by the Carlsberg Foundation (Grant no. CF20-0122).
- Reference system identifier
- WGS 1984
- Distribution format
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Name Version Text
1
- Distributor contact
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Organisation name Individual name Electronic mail address Role Institute of Oceanology Polish Academy of Sciences
Monika Grabowska
Custodian
- OnLine resource
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Protocol Linkage Name WWW:LINK-1.0-http--related
https://doi.org/10.1016/j.jcz.2024.10.013 Published article
WWW:LINK-1.0-http--related
https://www.ncbi.nlm.nih.gov/nuccore/?term=PQ272140:PQ272265[accn] NCBI GenBank
WWW:DOWNLOAD-1.0-http--download
https://opendap.iopan.pl/opendap/data/csv/DragonNest/dataset_kino_pop_gen_GL_24.csv dataset_kino_pop_gen_GL_24
- OnLine resource
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Protocol Linkage Name DOI
https://doi.org/10.48457/IOPAN.2025.503 Digital Object Identifier (DOI)
- Hierarchy level
- Dataset
- Statement
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The data was obtained, processed and published according to known and used scientific standards.
Metadata
- File identifier
- c6f12360-235a-464d-af8f-baaa179d4e0b XML
- Metadata language
- English
- Character set
- UTF8
- Hierarchy level
- Dataset
- Date stamp
- 2025-10-06T10:30:04.371771Z
- Metadata standard name
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ISO 19115:2003/19139
- Metadata standard version
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1.0
- Metadata author
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Organisation name Individual name Electronic mail address Role Institute of Oceanology
Aleksandra Zalewska
Publisher
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